补充材料国家电子邮

abdullahalamins · 发表于 2023-7-26 19:26:37

的药物和药物递送方法。许多研究人员正在做出前所未有的努力来开发新的基于 RNA 的药物。因此，随着RNA治疗技术的进步，未来有望开发出更加多样化的基于RNA的药物和药物递送方法。三种不同的HIF1A-As2 LNA GapmeR ASO。根据制造商的说明，使用 Hiperfect 试剂 (Qiagen) 转染 ASO (50 nM) 或 siRNA (50 nM) 48 小时。用Lipofectamine 2000试剂(ThermoFisher Scientific)将质粒瞬时转染至细胞中。siRNA、ASO 序列

节细胞生长或凋亡。集落形成测定表明，MYC 过表达损害了 H1299 和 CALU1 中HI亚洲手机号码列表F1A-As2 KD的集落形成（图 6F，补充材料 11C）。MYC KD 可以拮抗HIF1A-As2促进的 H1299 和 H460 集落形成（补充材料 11D、E）。在DHX9 KO实验中观察到类似的结果（图 6G）。此外，MYC 的过表达分别改变了HIF1A-As KD 或 DHX9 KO 所抑制的 3D 球体形成（补充图 12 ））。此外，Annexin-V 测定表明，MYC OE 或 p21 KD 损害了H1299

和 CALU1 中两种不同的HIF1A-As2 ASO诱导的细胞凋亡（件列表13）。总之，这些数据表明HIF1A-As2和 DHX9 通过 MYC 靶基因调节 NSCLC 中的细胞行为。 KRAS通过诱导 MYC激活HIF1A-As2 接下来，我们研究了 KRAS 在 NSCLC 中诱导HIF1A-As2的机制。我们注意到，MYC 基因特征在 KRAS WT 和 G12D 基因集中富集（图 7A）。异位KRAS W
T和G12D增加了H1299中的MYC水平（图 7B）并且KRAS与LUAD中的MYC呈正相关（图 7C）。一致地，MYC KD 降低了H1299 和 A549 细胞中HIF1A-As2 的表达，以MALAT1作为阳性对照（图 7D）[ 39 ]。这一观察结果表明，KRAS 的下游基因 MYC 可能参与KRAS 对HIF1A-As2的诱导。图 7：KRAS 通过 MYC 激活HIF1A-As2。图7 MYC基因特征在 KRAS ...查看全文

xelozz · 发表于 2024-5-8 16:22:01

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xelozz · 发表于 2024-5-8 16:23:04

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xelozz · 发表于 2024-6-2 12:39:40

xelozz · 发表于 2024-6-2 12:40:43

xelozz · 发表于 2025-6-15 10:38:24

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xelozz · 发表于 2025-6-15 10:39:29

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xelozz · 发表于 2025-8-15 00:21:49

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xelozz · 发表于 2025-8-15 00:22:55

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